BAC Shotgun Sequencing

Cambridge Bioscience offers access to a BAC shotgun sequencing service from Lucigen for the full length insert sequencing of large-insert cosmid, fosmid and BAC clones.

Service Features
• 95% or higher frequency of clones with inserts
• Exclusive pSMART shotgun library construction
• pSMART GC or triple-A vector capable of cloning inserts up to 10kb (typically 2-4kb)
• No chimeras
• Construction of libraries in custom plasmid vector available
• Fast turnaround time - ~1-2 weeks

Method
Purified DNA from the cosmid, fosmid or BAC clone is randomly sheared, end-polished, size-selected, and subcloned into the exclusive pSMART® Vector from Lucigen and transformed into the E. cloni® 10G competent cells. The colonies of the shotgun library are robotically picked into 96-well plates for high-throughtput DNA preparation and Sanger sequencing. This service includes large-insert DNA preparation (optional), shotgun subcloning, library construction, colony picking, overnight culturing, DNA preparation from clones, DNA sequencing at any scale from a single sample to a 96-well high-throughput processing.

Data Provided
DNA sequences are transmitted via secure internet connection or provided on appropriate computer media on project completion. A complete shotgun library is provided at no additional cost.

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