BAC End Sequencing
Cambridge Bioscience offers access to a BAC end sequencing service with universal primers to the BAC vector, or gene-specific primers (supplied by the client) from Lucigen.
This service typically produces an average of at least 90% success and reads >700bp for both forward and reverse directions. The service includes robotic colony picking, overnight bacterial cell culture, DNA extraction from clones and DNA sequencing at any scale from a single sample to 96- or 384-well high-throughput processing.
• Over 90% sequence success rate
• Over 700bp per read
• Robotic colony picking and overnight culturing
• Automated high-throughput DNA preparation
DNA sequences will be transmitted via secure internet connection or provided on appropriate computer media on completion of the project. Typically raw sequence data and/or auto-trimmed BAC end sequences in FAST/TX formats will be provided.
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