AQIX® Tissue Transport SolutionThe storage and transport of human tissues is an important part of physiological research and preclinical drug bioassay studies. However many commercially available storage and transport solutions have a formulation that reflects the intracellular rather than the required extracellular solution.
Cambridge Biosciences offers a GMP manufactured, chemically defined, animal origin-free physiologically balanced liquid medium called AQIX® RS-I for the storage and transport of human tissue.
AQIX® RS-I is a patented solution designed from first principles to simulate human interstitial fluid. This solution contains substrates known to maintain all aspects of cellular function including auto-regulatory mechanisms, the Krebs cycle and ADP:ATP balance replicating the essential, intercellular conditions surrounding each cell membrane creating a homeostatic environment. It has even been demonstrated that the glycolytic pathway is sustained by AQIX® RS-I under periods of ischemia and anaerobic metabolism. This ensures the viability and ultimate survival of isolated human cells, tissues and organs over hours and days
Benefits of Using AQIX® RS-I
AQIX® RS-I recreates the surrounding conditions of tissues, cells and organs. This creates a number of benefits not achieved by other solutions that can be applied to multiple applications.
• Cells, tissues & organs (animal & human) can be kept viable for significantly longer periods than previously achieved
• Cell, tissue & organ morphology is maintained
• Cell membrane receptors of all cell types are held in an optimal, sensitized state
• Cell membrane receptors react as they do in vivo
• Enzyme activity is maintained
• Neuronal activity & organ innervations is preserved
• Maintains potency preserving a cell’s ability to self replicate, expand and differentiate
• One single solution can be used for all mammalian species
Applications of AQIX® RS-I
AQIX® RS-I can be used in numerous applications including:
• Tissue & Organ Transport
• Tissue & Organ Preservation
• Tissue & Organ Perfusion
• Transplant Perfusion
Tissue & Organ Transport, Preservation & Perfusion
AQIX® RS-I has been successfully used for tissue and organ preservation and perfusion. It is suitable for a variety of cell, tissue and organ types, across all mammalian species utilized in physiological research and preclinical drug bioassay studies and has been successfully utilised in the following animal and human preparations:
• Vascular tissues
• Urino-genital tract
• Gastro-intestinal tract
• Skeletal & visceral neuromuscular preparations
• ES-derived stem cells
AQIX® RS-I Formulation
AQIX® RS-I was designed to only contain serum components at serum levels:
• Ionic Species & Levels
The concentrations of the ionic species in AQIX® RS-I acknowledges the activity coefficients of ionic species rather than just the total serum concentrations. This means that the serum binding of Ca2+ and Mg2+ is distinguished from the actual free, ionised levels of the ions. Therefore, the correct Na+/K+ balance retains the ionic milieu within the cell membrane and the correct Ca2+ buffering and Ca2+/Mg2+ ratio enable optimal membrane functions and biochemical mechanisms
• Metabolic Substrates
AQIX® RS-I contains all the substrates, such as aspartate, glutamate and glutamine, required to support intermediate metabolism. These substrates are contained at optimal serum levels to maximise the viability of cellular functions over time. This has been verified in 4,000 scientific trials on cells, embryos, tissues and organs from a variety of mammalian species.
• Buffering Media
AQIX® RS-I uses the natural pH buffering mechanism found in human serum, namely, pCO2 / HCO3 -, utilising a Good's buffer, BES. This buffer has pKa and zwitteronic characteristics that ensures pH stability over the temperature range 10°C -38 °C allowing optimal serum levels of bicarbonate ions [HCO3-] to be utilised.
• Oncotic/Colloidal Pressure
The formulation of AQIX® RS-I acknowledges of role of the ionic, osmolarity, pH, specific ion conductivity and substrate demands of mammalian tissues and organs. Chemical and biophysical analyses have shown that the serum composition in mammalian species is uniform from 'mouse to man' and AQIX® RS-I mimics this.