Product Category: PCR and DNA Cloning

Clone the Unclonable

Lucigen's pSMART vectors are a new generation of transcription and translation-free cloning vectors designed to eliminate cloning gaps commonly found with many plasmids.

• Gap-free cloning
• <1% background
• No post-ligation cleanup prior to transformation
• Small vector size (1.7-2.0kb)
• Ampicillin or kanamycin selection
• Pre-processed vectors eliminate vectro digestion, gel purification and dephosphorylation
• High efficiency cloning with either electroporation or chemically competent cells

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Ultra-fast cloning with Cold Fusion technology

System Biosciences Cold Fusion Cloning kits use a new and convenient method of DNA cloning. These kits are capable of joining single and multiple PCR fragment(s) into a linearised destination vector in a single step at room temperature.

• PCR product(s) rapidly and accurately fuse into the linearised vector in the desired orientation.
• 5 minute incubation at room temperature followed by 10 minutes on ice.
• Highly efficient system, with more than 95% positive cloning rate.
• Restriction enzyme, ligase and phosphatase free system.
• Competent cells included.
• Supports a broad range of fragment size.

 
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Big Easy Long PCR Cloning Kit

The BigEasy Long PCR Cloning Kits contain everything needed to efficiently clone PCR products up to 30 kb into an unbiased, high-fidelity cloning vector. There are two different versions of the kit that are compatible with either proofreading or non-proofreading PCR polymerases. They can also be used to clone any blunt or G-tailed DNA up to 30 kb.

• Perfect for long PCRs, large cDNAs, and 10-30 kb libraries.
• Highest stability cloning system known.
• Get every gene sequence.
• No insert size or content bias.