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Product Category: siRNA and microRNA

SBI's miRZip anti-microRNA Lentivectors

Download SBI’s latest miRZip Biotechniques Application Note.

miRZip™ anti-sense microRNAs are stably expressed RNAi hairpins that have anti-microRNA activity. The miRZip shRNAs produce short, single-stranded anti-microRNAs that competitively bind their endogenous microRNA target and inhibit its function. The result is the elevation of the protein levels of the transcripts targeted by the microRNA being “zipped”.

The miRZip short hairpin RNAs are cloned into SBI’s pGreenPuro™ shRNA expression lentivector. The miRZip hairpins are rationally designed for asymmetry such that the upper strand of the hairpin (shown in gray in the attached image) does not contain the endogenous microRNA sequence and the lower strand is preferred for producing anti-sense microRNAs (shown in green in the attached image) that are fully complementary to a specific microRNA target.

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•   Stable & permanent antimicroRNA expresssion from constitutive H1 promoter
•   Anti-sense microRNAs sequester specificc endogenous microRNAs
•   Select for positive expressing cells with GFP or Puromycin
•   Uncover phenotypes using microRNA inhibition
•   Perform phenotypic screens using pooled miRZip libraries

QuantiMir Small RNA Quantitation System

The QuantiMir™ RT Kit is used to generate PCR-ready cDNA from total RNA to measure any small RNA. This system is much more sensitive, quantitative and easier than Northerns or RNase protection experiments. Synthesize enough cDNA template for multiple measurements from a single RT reaction. Profile any small RNA including microRNAs, siRNAs and piRNAs.

Measure and profile microRNA expression levels from any tissue source
Validate the existence of predicted and newly discovered microRNA species
Sensitive down to 10 pg or less of total starting RNA
A single tube, 3 step process completed in 2 hours
Rapidly tag and convert all small RNAs into detectable cDNA for thousands of qPCR measurements

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This technology is also available combined with pre-formatted microRNA qPCR assay panels for Cancer microRNAs (OncoMir qPCR Array) and for Stem Cell differentiation-associated (Stem Cell qPCR Array) microRNAs. SBI also offers genome-wide microRNA expression profiling qPCR assay sets.

QuantiMir

HuSh 29-mer shRNA

HuSh 29-mer hairpin expression clones appear to enter the RNAi pathway more efficiently and result in much higher potency and specificity than shorter expressed or oligo RNAi forms. shRNA less than 30 base pairs in length evades the radar of the mammalian interferon response and initiates strong and specific gene silencing. Please click here for the HuSh 29-mer Application Guide.

The OriGene HuSh 29-mer product line contains shRNA expression vectors offering genome wide coverage for human, mouse and rat species.

29mers are up to 100x more efficient than 21mers
Supplied as 4 constructs in purified plasmid
Empty vector and scrambled sequence controls provided
Choice of 3 retroviral vectors (untagged, GFP or RFP)
Transient or stable transfection or retroviral infection options
Guaranteed successful knockdown

Please click here to request pricing information or more details on HuSh 29-mer products.

Price information can be downloaded from the following links:

Human HuSh 29mer, GFP Human HuSh 29mer and RFP Human HuSh 29mer.

TransIT-TKO siRNA Transfection Reagent

TransIT-TKO is a high efficiency, low toxicity, siRNA transfection reagent for mammalian cells.

Broad Spectrum siRNA Delivery - Utilize one transfection reagent and protocol for a wide variety of cells.

Low cellular toxicity
Maintain cell density and reduce experimental biases.
Reproducible results
Obtain consistent, targeted gene knockdowns from day to day.
High knockdown efficiency
Achieve optimal gene silencing in a large percentage of cells to ensure experimental success

TransIT-TKO

Full Spectrum Global microRNA Amplification Kit

The Full Spectrum Global microRNA Amplification Kit enables the global amplification of small RNAs. Acheive 10,000 fold amplification of your micro RNAs

No purification steps needed
Amplify RNAs from 20-500 nucleotides in length
Sensitivity as low as 5ng total RNA for qRT-PCR
Generate target for a miRNA microarray from 100ng RNA
Positive control RNA included in the kit

Click here for a schematic diagram of how the kit works

Full Spectrum Global microRNA Kit

microRNA Discovery Kit

Rapid identification of new microRNAs and microRNA like molecules

Only 0.5 µg of starting total RNA without size selection
Amplification and cloning in a single day
Identify members of two new classes of small non-coding RNAs including the endogenous siRNAs and piRNAs
Both precursor and mature MicroRNAs can be identified

microRNA Discovery Kit

GenoExplorer microRNA Quantitative RT-PCR

Developed by Genosensor, The GenoExplorer miRNA quantitative RT-PCR kit has been developed and optimized to amplify specific microRNAs, allowing real time quantitation of miRNA expression levels.

Sensitive detection requires only 1-10ng total RNA or equivalent Specific single nucleotide discrimination
Available for both mature and precursor miRNAs
Endpoint or Real Time PCR

GenoExplorer RT-PCR Kit

GenoExplorer microRNA Quantification System

GenoExplorer microRNA array products enable you to perform miRNA assays and analyses in your laboratories. The microarray platform is flexible and compatible with most hybridization chambers and microarray scanners.

The products are highly sensitive and reproducible, and provide the latest updated miRNA information
Quantitative measurement of mature and precursor microRNAs
Customized chips for novel predicted miRNA Latest updated known miRNAs

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