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Product Category: Fluorescent Labelling and Imaging

Mix-n-Stain™ CF™ Dye Antibody Labelling Kits

We are delighted to offer the revolutionary new Mix-n-Stain™ CF™ Dye Antibody Labelling Kit from our partner Biotium.

With 16 dye options spanning absorbances of 350nm to 770nm and less than 30 seconds hands on time, this kit will dramatically simplify the process of preparing fluorescently labelled antibodies producing a conjugate ready for microscopy, immunofluorescence, flow cytometry or Western blotting.

Tto find out more, please Click Here

New Fluorescent Protein & Fluorescent Protein Antibody Sets

We are pleased to offer a new Fluorescent Protein set and a new fluorescent protein antibody set from our supplier BioVision. Fluorescence proteins have become well established markers for gene expression detection studies and protein targeting in intact cells and organisms.

The fluorescent protein set contains 6 of the brightest E. coli Green, Red, mCherry, Blue, Cyan and Yellow fluorescent proteins (EGFP, dsRed, mCherry, BFP, CFP & YFP). The fluorescent proteins have been purified to a high purity and exhibit higher fluorescence than other similar fluorescent proteins on the market. The presence of the His-tag on the majority of these proteins allows their detection by both His-tag antibody and specific fluorescent protein antibodies. We are pleased to offer the matching fluorescent protein antibody set in association with these proteins.

Blue Fluorescent Protein TagBFP

TagBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.

  • Monomeric fast maturing protein with successful performance in fusions
  • Extremely high pH-stability and photostability
  • Recommended as a donor for green fluorescent proteins in FRET applications

click here to visit the Evrogen supplier page


Biological Application of FRET – Protease Detection

In recent years, FRET based assays have found broad applications, one of which is the detection of protease activity. Proteases play key roles in protein activation, cell regulation and signaling, as well as in the generation of amino acids for protein synthesis or utilization in metabolic pathways.

FRET-based assays have made the detection of protease activity and High Throughput Screening of protease inhibitors faster and easier. AnaSpec, a world leader in FRET peptide technology, is proud to be the first in the industry to offer long wavelength protease assays for:

Cathepsins D, S
HCV (Hepatitis C Virus)
HIV
MMPs
α- (TACE), ß-Secretase (BACE1)
Renin
and WNV (West Nile Virus)

Please click here for a FRET technology Guide


Label-IT Nucleic Acid Labelling Reagents

Label Any DNA or RNA Template using LabelIT nucleic acid labelling reagents.. The LabelIT method is efficient, diretct, non-enzymatic labelling

Suitable for a wide range of applications

  •  One-step Chemical Method—Easily and consistently control the labeling reactions.
  • Adjustable Labeling Density—Achieve high sensitivity with optimally labeled DNA and RNA.
  • Covalent Mechanism—Permanent, non-destructive modification of nucleic acid residues is ideal for many diverse applications; labels do not impact hybridization performance.

 click here for further informtion

New GelRed™ & GelGreen™ Prestaining Kits

If you are looking for a safe alternative to Ethidium Bromide, without compromising on performance, then you should try GelRed™ or GelGreen™ nucleic acid stains. These gels stains are widely recognised as the best alternatives to Ethidium Bromide for the following reasons:
  • Unrivalled sensitivity
  • Cell membrane impermeability for true safety. Impenetrable to latex gloves
  • Compatible with all downstream manipulations
  • Extremely stable to both heat and light
  • Compatible with existing instruments
The products have recently been updated to a new convenient and efficient Prestaining Kit format. More information can be found here.

HiLytePlus Long Wavelength Dyes

AnaSpec is pleased to introduce HiLytePlusTM. Built on HiLyte FluorTM technology, the HiLytePlus series represents AnaSpec's highest performing dyes ever for long wavelengths.

HiLytePlus Series

  • Even brighter than HiLyte Fluor dyes
  • Like HiLyte Fluor series, outperforms Cy® & DyLight dyes
  • Superior photostability
  • Excitation wavelengths of 555, 647 and 750 nm
  • Ideal for high-performance biomarker labeling

  • Duolink Detection Kit HRP/NovaRED

     

    Duolink Detection Kit HRP/NovaRED

    Use brightfield microscopy to detect and quantify endogenous proteins, protein modifications and protein interactions in tissue sections after performing Duolink® in situ PLA™ technology.

    • Investigate protein interaction events in tissue

    • Quantify and standardize data interpretation

    • Obtain clear, high resolution imagery

    The Duolink Detection Kit HRP/NovaRED generates discrete spots that each represents individual proteins or protein events. The spots can automatically be counted, thereby bringing objectivity to investigations which have previously relied on subjective evaluation.

    Combine your own pair of primary antibodies with Duolink secondary reagents and Detection Kit HRP/NovaRED to elucidate the in situ localization and abundance of proteins or protein events. Work on archived tissue material to reveal more information than your standard immunohistochemistry assay.

    Please click here for a copy of the instruction manual.

       
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