Tel: +44 (0)1223 316 855
life science research products, biological research products, biotechnology
close ...


Supplier:
Product types:
Electrocompetent Cells With The Highest Transformation Efficiency
Competent Cells Eliminating Endotoxin At Source
Enzyme-free, Directional Cloning & Expression Systems

Highest Transformation Efficiency Electrocompetent Cells

Highest Transformation Efficiency Electrocompetent Cells

Cambridge Bioscience offers the highest transformation efficiency electrocompetent cells on the market (≥ 4 × 1010 cfu/µg pUC DNA). The E. cloni® 10G SUPREME electrocompetent cells are ideal for the most demanding cloning situations, including the construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.

Benefits of Using the E. cloni® 10G SUPREME Electrocompetent Cells
• Highest transformation efficiency on the market - ≥ 4 × 1010 cfu/µg pUC DNA
• High yield optimised
• Direct replacement for standard cloning strains - DH5α™, DH10B™, JM109, TOP10, XL1-Blue
• Ideal for the most demanding cloning situations - construction of large, high complexity libraries & cloning difficult targets
• Convenient packaging options

Features of the E. cloni® 10G SUPREME Electrocompetent Cells
• Phage T1 resistant
• Endonuclease & recombination minus
• Blue/white screening-capable
• Packing options:
- SOLOs: Aliquots of 25 μl individual transformations
- DUOs: Aliquots of 50 μl for two transformation reactions of 25 μl each

Transformation Efficiency Comparison

Transformation Efficiency
Transformation efficiency comparison of E. cloni® 10G SUPREME cells (Lucigen) and Mega DH10B cells (Invitrogen). Competent cells transformed according to manufacturer’s instructions with same pUC19 DNA control (provided with the Invitrogen cells)

Recombinant Yield Comparison

Recombinant Yields
E. cloni®10G SUPREME electrocompetent cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).

E. cloni® Comparison Table for Cloning & Library Construction

E. Cloni
E. cloni® Range
Find out more about the complete range of E. cloni® cells Here

Material Available For Download
E. cloni® 10G & 10GF´ Electrocompetent Cells Manual

DH5α™ & DH10B™ are trademarks of Life Technologies Inc

Products

Note: availability depends on country - see product detail page.

Contact Our Lucigen Specialist, Katie