Carbamylation is a post-translational modification resulting from the binding of isocyanic acid, spontaneously derived from high concentrations of urea which occurs throughout the lifespan of proteins in vivo. The carbamylation of proteins is usually associated with a partial or complete loss of protein function. It has been shown that elevated urea directly induces the formation of potentially atherogenic carbamylated LDL (cLDL). High blood concentrations of urea leading to the carbamylation process have also been detected in uremic patients and patients with end-stage renal disease.

Cambridge Bioscience offers the OxiSelect™ protein carbamylation ELISA for the rapid detection and quantitation of protein carbamylation from Cell Biolabs. This kit is compatible with cell lysates, blood samples and purified proteins and has a detection sensitivity limit of 4 ng/mL of CBL-BSA.

Assay Principle
CBL-BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC. The CBL protein adducts present in the sample or standard are probed with an anti-CBL antibody, followed by an HRP conjugated secondary antibody. The protein CBL adduct content in an unknown sample is determined by comparing against a standard curve prepared from CBL-BSA standards.

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