Calcium Detection via the Aequorin Complex
The aequorin complex (coelenterazine & apoaequorin) has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from ~0.1 uM to >100 uM using native coelentrazine.
Cambridge Bioscience offers both native coelentrazine and a selection of synthetic coelentrazine analogues for detecting calcium. Each of the synthetic derivatives boasts a unique sensitivity resulting from generation of 3% to 190 times the brightness of native coelentrazine. Uniquely among these, coelentrazine e enables the ratiometric quantification of Ca2+ levels through its dual emission peaks.
Luminescent Properties of Coelenterazine Products with Apoaequorin*
Cambridge Bioscience offers each of these substrates separately and also as part of the sampler kit containing 25ug aliquots of the coelentrazine native, cp, f, fcp, h, hcp, i, ip & n analogues at greater than 98% purity. In addition Coelentrazine (native) and Coelentrazine h are also available in special formulations readily soluble in water and PBS.
Note: product availability depends on country. Product detail pages show availability of each product.