Intracellular ROS Assay
Oxidative stress is a state in which the production of free radicals outpaces the clearance leading to accumulation and oxidative damage to proteins, DNA and lipids. Oxidative damage has been linked to a variety of diseases such as ageing, asthma, arthritis, cardiovascular disease, atherosclerosis, Down’s syndrome and neurodegenerative diseases.
Cambridge Bioscience offers the OxiSelect™ intracellular ROS assay kit from Cell Biolabs. The OxiSelect™ Intracellular ROS Assay Kit is a fluorometric, cell-based assay for measuring hydroxyl, peroxyl and other reactive oxygen species activity within cells. This assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is converted into the fluorescent DCF detectable in a two-step process that can be detected using a standard fluorescence plate reader. The kit boasts excellent sensitivity, a simple and fast protocol and has been used as part of research in several published articles.
Benefits of the OxiSelect™ Intracellular ROS Assay
• Fast - Quick 1 hour workflow
• Highly sensitive - down to 10pM
• Wide detection - Detects the presence of various ROS species
• Tried & tested kit - cited in numerous publications
OxiSelect™ Intracellular ROS Assay Workflow
1. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells
2. DCFH-DA is de-acetylated to DCFH by cellular esterases
3. In the presence of ROS, DCFH is subsequently rapidly oxidised to highly fluorescent DCF
To find out more about this kit (PDF), please Click Here
The OxiSelect™ intracellular ROS assay has been cited in numerous publications. Please click on the links below to find out more.
1. Cho, K.A. et al. (2012). IL-17 and IL-22 Enhance Skin Inflammation by Stimulating the Secretion of IL-1ß by Keratinocytes via the ROS-NLRP3-Caspase-1 Pathway. Int. Immunol. 10.1093/intimm/dxr110.
2. Martin, S.A. et al. (2011). Parallel High-Throughput RNA Interference Screens Identify PINK1 as a Potential Therapeutic Target for the Treatment of DNA Mismatch Repair-Deficient Cancers. Cancer Res. 71:1836-1848.
3. Kuznetsov, J.N. et al. (2011). AMPK and Akt Determine Apoptotic Cell Death following Perturbations of One-Carbon Metabolism by Regulating ER Stress in Acute Lymphoblastic Leukemia. Mol. Cancer Ther. 10:437-447.
4. Wei, J. et al. (2011). C-Jun N-terminal Kinase (JNK-1) Confers Protection Against Brief but not Extended Ischemia during Acute Myocardial Infarction. J. Biol. Chem. 286:13995-14006.
5. Titos, E. et al. (2011). Resolvin D1 and its Precursor Docosahexaenoic Acid Promote Resolution of Adipose Tissue Inflammation by Eliciting Macrophage Polarization toward an M2-Like Phenotype. J. Immunol. 187:5408-5418.
6. Zhang, J. et al. (2011). 2-Deoxy-D-Glucose Attenuates Isoflurane-Induced Cytotoxicity in an In Vitro Cell Culture Model of H4 Human Neuroglioma Cells. Anesth. Analg. 113:1468-1475.
7. Zhang, Y. et al. (2010). The Mitochondrial Pathway of Anesthetic Isofluorane-Induced Apoptosis. J. Biol. Chem. 285:4025-4037.
8. Batova, A. et al. (2010). The Synthetic Caged Garcinia Xanthone Cluvenone Induces Cell Stress and Apoptosis and Has Immune Modulatory Activity. Mol. Cancer Ther. 9:2869-2878.
9. Tanaka, N. et al. (2010). Cis-dichlorodiammineplatinum Upregulates Angiotensin II Type 1 Receptors through Reactive Oxygen Species Generation and Enhances VEGF Production in Bladder Cancer. Mol. Cancer Ther. 9:2982-2992.
Other ROS Assays
Cambridge Bioscience also offers an in vitro ROS/RNS assay and a hydrogen peroxide and hydrogen peroxide/peroxidase assay. To find out more, please Click Here
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