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TransIT®-2020: High Performance Transfection

TransIT®-2020: High Performance Transfection

Cambridge Bioscience offers the broad spectrum, high-performance, animal-origin free, serum compatible DNA transfection reagent, TransIT®-2020 from Mirus Bio. TransIT®-2020 enables transfection of plasmid DNA into mammalian and insect cells providing high levels of gene expression in hard-to-transfect cell types including primary and stem cells.

Benefits of Using TransIT®-2020
Broad spectrum DNA delivery
Enables one transfection reagent and one protocol to be used for the transfection of mammalian and insect cells including hard-to-transfect primary and stem cells
Superior transfection of insect cells
Obtains higher expression than other insect cell transfection reagents
Transfects adherent and suspension cells
Suitable for the transfection of adherent and suspension cells
Outperforms competitor reagents
Demonstrates higher protein yield when compared to FuGENE® HD, Lipofectamine™ 2000, and Lipofectamine™ 2000 CD
Animal origin free
Provides high performance with maximum compatibility
High efficiency delivery
Achieves expression in a large population of cells for experimental success
Stable & transient transfection
Suitable for both stable and transient transfection
Serum compatible
Proprietary lipid and protein/polyamide formulation enables efficient transfection in the presence of serum eliminating the need for any culture medium change after transfection

Customer Testimonial
“Our lab routinely works with primary human fibroblasts which are notoriously difficult to transfect, and we require a method that is non-toxic, reliable, and reproducible. TransIT-2020 is the only reagent on the market that fits those criteria. In addition, TransIT-2020 also transfects colon cancer cell lines HCT-116 and DLD1 with much higher efficiencies. Overall, the pace and quality of our research have improved since we started using TransIT-2020.”
John A. Burns
David Scicchitano Lab, New York University – Department of Biology

Request A Free Sample
To request a free sample of TransIT®-2020, please Click Here

TransIT®-2020 Produces Superior Gene Expression in a Broad Spectrum of Cell Types

TransIT-2020_Cell_Types
The indicated cell types were transfected in 96-well plates with a luciferase expression plasmid (0.1ug/well) according to the manufacturer’s protocol. Reagent to DNA ratios were optimized for each cell type: TransIT®-2020 (Mirus Bio, 2:1 or 3:1), FuGENE® HD (Roche, 3.5:1), Lipofectamine™ 2000 (Life Technologies, 1.5:1, 3:1 or 5:1). Luciferase activity was measured 24 hours post-transfection. Values were normalized to TransIT-2020 and presented as a percentage of luciferase expression.

TransIT®-2020 Reagent Exhibits Higher Expression & Lower Cellular Toxicity Compared to Other Transfection Reagents
TransIT-2020_Toxicity
Human umbilical vein endothelial cells (HUVEC) were transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph. Error bars represent the standard deviation of triplicate wells.

TransIT®-2020 Reagent Effectively Transfects Drosophila S2 Cells

TransIT-2020_Insect_Cells
Cells were transfected with a plasmid construct expressing a secreted form of the Dscam extracellular domain fused to alkaline phosphatase (AP) in a 24-well plate. The ratio of transfection reagent to DNA and micrograms of DNA per well is noted beneath each bar. All products were used according to manufacturers’ protocol. All transfections were performed in serum-free media for four hours followed by complete media supplementation. An AP enzymatic assay was used to measure the AP levels 24 hours post-transfection

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