Cambridge Bioscience offers the broad spectrum, low toxicity, serum-compatible DNA transfection reagent, TransIT®-LT1 from Mirus Bio. TransIT®-LT1 provides an efficient and cost effective transfection option for researchers who work with a range of different cell types including primary cells.

Benefits of Using TransIT®-LT1
• Broad spectrum DNA delivery
Enables one transfection reagent and one protocol to be used for the transfection of a variety of cells including primary cells
• Low cellular toxicity
Maintains cell density and reduces experimental bias
• Transfects adherent and suspension cells
Suitable for the transfection of adherent and suspension cells
• High efficiency delivery
Achieves expression in a large population of cells for experimental success
• Delivers single or multiple plasmids
Suitable for many applications such as gene expression, shRNA expression, viral production and promoter analysis
• Stable & transient transfection
Suitable for both stable and transient transfection
• Serum compatible
Proprietary lipid and protein/polyamide formulation enables efficient transfection in the presence of serum eliminating the need for any culture medium change after transfection

Customer Testimonial
"Our lab regularly transfects head and neck cancer squamous carcinoma cell lines as well as pre-cancerous oral lesion cell lines and primary cells with Mirus Bio products. We recently published a paper using Mirus Bio TransIT-LT1 in which we concluded tobacco carcinogens up-regulate AP-1 activity and AP-1 dependent IL-8 and VEGF gene expression in head and neck cancer."
Swenson, W et al. Molecular Carcinogenesis. April 2011
Beverly Wuertz
University of Minnesota - F. Ondrey Lab

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TransIT®-LT1 Efficiently Delivers DNA to a Wide Variety of Cell Lines
Using the TransIT®-LT1 Transfection Reagent, cells were transfected with the pEGFP-C1 expression vector, and the percentage of EGFP expressing cells was determined 24-48 hours post-transfection.

TransIT®-LT1 Reagent Exhibits Higher Expression & Lower Cellular Toxicity Compared to Other Transfection Reagents
HepG2 cells were transfected with a luciferase expression plasmid using the designated reagents at the manufacturer’s recommended reagent-to-DNA ratio indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph.

TransIT®-LT1 Reagent Performs Best in the Presence of Serum Containing Media
Using the TransIT®-LT1 Reagent, the indicated cells lines were transfected with a luciferase expression vector using three different media conditions: cells were transfected in the presence of (1) serum-free media or (2) complete media with media changes 4 hours post-transfection, or transfected in the presence of (3) complete media with no media change post-transfection. The cells were harvested 48 hours post-transfection, and the total luciferase activity per well was determined.

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