The CF™ dyes rival or exceed the brightness of other commercially available dyes such as Alexa Fluor® & Cy® dyes and are highly pH insensitive whilst boasting amongst the highest photostability available.

Cambridge Bioscience offers these dyes functionalised with hydrazide or oxyamine reactive groups to allow for the labelling of glycoproteins and other carbohydrate molecules.

Hydrazide & Oxyamine Reactive Dyes
The aminooxy group reacts with an aldehyde or ketone group to form a stable oxime linkage in an aqueous solution under neutral condition. The reaction kinetics are fast and can be further accelerated by adding aniline as a catalyst. Hydrazide reagents also react with aldehydes or ketones, but by contrast form unstable hydrazone linkages.

Benefits of Using CF™ Dyes
• Superior Brightness & Photostability
Rhodamine is known for its excellent photostability and brightness. Consequently many commercially available synthetic dyes use rhodamine as a base. Traditionally it has been impossible to modify rhodamine for use in the far-red and near-IR spectrum. However Biotium has overcome this challenge to develop rhodamine-based dyes in this spectral region with the important advantages of improved brightness and photostability.

• High Specificity
Many of the CF™ dyes are less highly charged than many other commercially available synthetic dyes such as Alexa Fluors®. Consequently in bioconjugates they have a reduced impact on the protein isoelectric point. This characteristic makes them ideal as antibody conjugates as they interfere less with target affinity and specificity.

CF™ Selection Guide
To view the full CF™ selection guide (PDF), please Click Here

Alexa Fluor® is a registered trademark of Invitrogen
Cy™ is a trademark of GE Healthcare

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