CF™488A is a green fluorescent dye from Biotium that offers similar brightness to Alexa Fluor® 488 whilst reducing spillover into the red channel. However its main advantage is that antibodies conjugated to CF™488A tend to have greater target specificity than those labelled with Cy™2 or Alexa Fluor® 488. CF™488A is less highly charged than Alexa Fluor® 488 and therefore does not distort the protein isoelectric point and affinity, increasing the signal to noise ratio.

Technical Details
Abs/Em Maxima: 490/515 nm
Extinction Coefficient: 70,000
Molecular Weight: ~710
Excitation Laser Line: 488 nm
Direct Replacement For: Alexa Fluor® 488, DyLight™488, fluorescein (aka FITC, FAM) & Cy™2

Staining Specificity Comparison

Cryosections of human heart stained with rabbit anti-fibronectin followed by goat anti-rabbit IgG conjugated to Cy2, Alexa Fluor 488, and CF488A, respectively. Courtesy of Dr. Sawa Kostin at Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut) in Bad Nauheim, Hessen, Germany.

Advantages of Using CF™488A
• Antibody conjugates show increased sensitivity over competitors
• High photostability
• Water soluble & pH insensitive
• Reduced spillover into red channel compared to Alexa Fluor® 488

CF™ Dye Labelled Secondary Antibodies
Cambridge Bioscience offers a comprehensive range of CF™ dye labelled secondary antibodies. To find out more, please Click Here

Mix-n-Stain™ CF™ Dye Antibody Labelling Kits
Cambridge Bioscience offers the Mix-n-Stain™ CF™ dye antibody labelling kits for the preparation of fluorescently labelled antibodies. To find out more, please Click Here

CF™ Dye References
To view a list of publications referencing the CF™ dyes, please Click Here

Alexa Fluor® is a registered trademark of Invitrogen
DyLight™ is a trademark of Thermo Fisher Scientific Inc.
Cy™ is a trademark of GE Healthcare

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