The Direct-zol™ RNA kits from Zymo Research enable the quick, purification of high-quality total RNA directly from TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, RNA-Bee™ and similar acid-guanidinium-phenol based reagents. These kits can purify up to 100 µg of high-quality total RNA with just seven minutes of preparation time and without phase separation or precipitation.
Benefits of using Direct-zol RNA kits
• Consistent and efficient purification of small and large RNAs
• DNA-free RNA produced
• Seven minute preparation time
• Phenol carryover eliminated
• No phase separation, precipitation or post purification
• Wide sample compatibility
• Compatible with most RNA isolation reagents
Direct-zol RNA kit formats available
Product | Catalogue number | Binding capacity | Format | Elution volume | Cells | Tissue |
---|---|---|---|---|---|---|
Direct-zol RNA Microprep | R2061 | 10 µg | Spin-column | 6 µl | ≤1x10 6 | ≤5 mg |
Direct-zol RNA Miniprep | R2051 | 50 µg | Spin-column | 25 µl | ≤5x10 6 | ≤25 mg |
Direct-zol RNA Miniprep Plus | R2071 | 100 µg | Spin-column | 50 µl | ≤1x10 7 | ≤50 mg |
Direct-zol-96 | R2055 | 10 µg | 96-well plate | 10 µl | ≤1x10 6 | ≤5 mg |
Direct-zol-96 MagBead | R2101 | 10 µg | Magnetic beads | 50 µl | ≤1x10 6 | ≤5 mg |
Direct-zol workflow vs traditional and competitor methods
Direct-zol™ | Trizol method | Supplier A miRNA kit | Supplier Q phenol kit | |
---|---|---|---|---|
Time per prep | 7 min | 90+ min | 30 min | 30 min |
No phase separation | ✓ | x | x | x |
NGS-grade | ✓ | x | x | x |
Small RNA recovery | ✓ | x | ✓ | x |
DNase I included | ✓ | x | x | x |
Obtain next generation sequencing (NGS) ready RNA with Direct-zol
High quality broad range RNA purified with Direct-zol
DNA-free RNA purified from human epithelial cells using Direct-zol compared to a DNA containing preparation from Supplier Q (1% agarose/TAE). Small RNAs were effectively recovered with Direct-zol™ while absent in Supplier Q preparations (Agilent Bioanalyzer 2100, Small RNA Chip data shown) (green).
Material available to download
Direct-zol Microprep protocol
Direct-zol Miniprep protocol
Direct-zol Miniprep Plus protocol
Direct-zol -96 RNA kit protocol
Direct-zol -96 Magbead kit protocol
Direct-zol RNA kits datasheet
Free samples
Request a free Direct-zol RNA Miniprep kit sample
TRI Reagent, TRIzol and RNAzol are registered trademarks of Molecular Research Center, Inc. QIAzol is a registered trademark of QIAGEN GmbH. TriPure is a trademark of Roche Diagnostics Operations, Inc. RNA-Bee is a trademark of Tel-Test, Inc.
Products
Note: product availability depends on country - see product detail page.