EvaGreen® is a green fluorescent nucleic acid dye ideal for qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), routine solution DNA quantification and capillary gel electrophoresis. This dye is compatible with instruments equipped with the 488nm argon laser or any visible light excitation with wavelength in this region due to its excitation and emission spectra when DNA-bound. Unlike other dyes, EvaGreen® is extremely stable, nonmutagenic and noncytotoxic by being completely impermeable to cell membranes.

Quantitative Real-Time PCR
The unique properties of EvaGreen dye have made it particularly useful in quantitative real-time PCR (qPCR) application. Compared with widely used PCR dyes, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen dye can be used at a much higher dye concentration, resulting in more robust signal for both PCR and melt curve analysis.

Benefits of Using EvaGreen®
• Low PCR Inhibition
Exhibit much less PCR inhibition than other PCR dyes via a smart "release-on-demand" DNA-binding technology
• Highly Sensitive
Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals (Practicing HRM may require a license from Idaho Technologies, Inc)
• Nonmutagenic & Noncytotoxic
Nonmutagenic and noncytotoxic by standard Ames test and completely impermeable to cell membranes
• Thermostable, Hydrolytically Stable & Photostable
• Spectrally Similar to other PCR dyes
Compatible with all major brand qPCR instruments and enzyme systems
• Compatible with Fast PCR Protocol
Minimal interference to PCR makes it possible to significantly shorten the chain extension time
• Compatible with Multiplex PCR
No dye migration from amplicon to amplicon when used at the recommended concentration

Cambridge Bioscience offers a range of products using EvaGreen®.

Fast EvaGreen® qPCR Master Mix
Fast Evagreen® qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses both the EvaGreen® qPCR dye and the Cheetah Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.

Fast-Plus EvaGreen® qPCR Master Mix
The Fast-Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. This master mix contains the safe EvaGreen® dye and the fast-activating chemically-modified hotstart enzyme Cheetah™ Taq. EvaGreen® binds to dsDNA via a novel “release-on-demand” mechanism permiting saturation dye concentration in qPCR without PCR inhibition. This mix is available in three formats containing different amounts of ROX reference dye for use in different PCR instruments.

Products

Note: product availability depends on country. Product detail pages show availability of each product.