miRNA Based iPS Cell Generation
The discovery that somatic cells could be induced to a pluripotent state via the introduction of the Oct4, Sox2, Klf4 & Myc transcription factors created a valuable new source of embryonic stem cell like cells ideal for drug discovery and basic research. However, a recent publication has shown that miRNA based reprogramming methods can be far more efficient than traditional methods*.
Cambridge Bioscience is pleased to offer new and innovative miRNA based reprogramming vectors for iPS cell generation developed by System Biosciences. These lentiviral and minicircle miRNA based vectors contain the 302bcad/367 miRNA cluster to induce both human and mouse somatic cells into a pluripotent state.
miRNA-based Reprogramming Method
The miRNA-based reprogramming technique has been recently shown to have a dramatically higher reprogramming efficiency than current Oct4/Sox2/Klf4/Myc transcription factor based reprogramming. Reprogrammed cells produced by this method show typical iPS characteristics including morphology, marker expression and capacity for teratoma formation. Additionaly, the mouse miR-iPS cells are able to contribute to successful chimera germline transmission.
Benefits of using miRNA based Reprogramming
• Dramatically more efficient than current techniques
• Available in lentiviral particle and non-integrating minicircle construct formats
• Convenient ready to transfect or transduce format
Formats
This miRNA based system is available in two formats:
1. Lentiviral Vector Format
The lentiviral vector format uses the traditional lentiviral expression system integrating the viral DNA to generate miRNA constructs for cell reprogramming. These vectors are available as transduction ready pre-packaged virus particles with GFP or GFP and puromycin markers.
To view the lentiviral transduction protocol (PDF), please Click Here
2. Minicircle Plasmid Format
Minicircle DNA is an easy-to-transfect non-viral, non-integrating plasmid that has an extended expression lifespan in cells enabling efficient reprogramming of somatic cells to the pluripotent state. This format is ideal for non-viral iPSC generation.
To view the minicircle transduction protocol (PDF), please Click Here
Landmark Publication
To view the landmark publication related to this product, please click below*:
Anokye-Danso F, Trivedi CM, Juhr D, Gupta M, Cui Z, Tian Y, Zhang Y, Yang W, Gruber PJ, Epstein JA, Morrisey EE. Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency. Cell Stem Cell. 2011 Apr 8;8(4):376-88.
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