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Large & Difficult To Clone Fragment Cloning Kits

Large & Difficult To Clone Fragment Cloning Kits

Cambridge Bioscience offers large and difficult to clone fragment cloning kits from Lucigen. The BigEasy Kit with the pJAZZ vector is ideal for constructing bias-free, large-insert genomic libraries, or for cloning difficult DNA of any size up to 30 kb. The novel pJAZZ vector is maintained as a linear molecule with ends of the vector that can rotate freely and therefore does not supercoil. Without the torsional stress induced by supercoiling, otherwise unclonable sequences (e.g., repetitive, or A/T-rich or G/C-rich sequences) are stabilized. The pJAZZ linear cloning vector is maintained at low copy number (5-10/cell) in BigEasy-TSA™ Electrocompetent Cells (>4 x 1010 cfu/µg), which are required for transformation and propagation. The copy number can be induced 5-10X in this strain.

The BigEasy Long PCR Cloning Kits contain everything needed to efficiently clone blunt or G-tailed PCR products up to 30 kb into the unbiased, high-fidelity pJAZZ linear cloning vector. There are two different versions of the vector (Figure 2) that are compatible with either proofreading (pJAZZ-OK Blunt Vector) or non-proofreading (pJAZZ-OK GC Vector) PCR polymerases.

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