Large & Difficult To Clone Fragment Cloning Kits
Cambridge Bioscience offers the large and difficult-to-clone fragment BigEasy® v2.0 linear cloning system. Ideal for constructing bias-free, large-insert genomic libraries, or for cloning difficult DNA of any size up to 30 kb, the linearised pJAZZ vector prevents torsional stress caused by supercoiling, stabilising otherwise unclonable sequences.
Benefits of Using the BigEasy® v2.0 Linear Cloning System
• Maximum insert stability
• Efficiently clone any insert up to 30 kb
• Create libraries from A/T-rich or G/C-rich genomes
• Clone gene clusters or operons
• Inducible copy number
BigEasy-TSA™ Electrocompetent Cells
The pJAZZ linear cloning vector is maintained at low copy number (5-10/cell) in BigEasy-TSA™ electrocompetent cells (>4 x 1010 cfu/µg), which are required for transformation and propagation. The copy number can be induced 5-10X in this strain.
The BigEasy® long PCR cloning kits contain everything needed to efficiently clone blunt or G-tailed PCR products up to 30 kb into the unbiased, high-fidelity pJAZZ linear cloning vector.
1. Proofreading PCR polymerases (pJAZZ-OK Blunt Vector)
2. Non-proofreading PCR polymerases (pJAZZ-OK GC Vector)
Note: availability depends on country - see product detail page.